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为了在分子水平上研究拟除虫菊酯抗性家蝇的 P4 50基因的特性 ,更好地研究其与抗性相关的机制 ,本研究根据在美洲地区的抗性家蝇品系 ( LPR)克隆的 P4 50基因 MDC YP6D1的保守序列设计合成引物 ,用 PCR方法从北京地区的家蝇中也扩增出 1条约 2 1 0 bp的特异性片段 ,片段长度与原序列对应片段相近 ,说明北京地区的家蝇 P4 50基因与 MDCYP6D1间有很大的同源性。H inf I酶切发现扩增产物上并没有相应的酶切位点 ,说明扩增产物与原序列 MDCYP6D1的相应部分不一样 ,提示这一用 PCR方法在北京地区的家蝇中扩增出的 P4 50基因片段有可能对应一新的 P4 50基因。 PCR-SSCP结果显示扩增产物在一级结构上有一定的多态性 ,这一现象可能与 P4 50基因在昆虫体内的复杂多样性有关
In order to study the characteristics of the P450 gene of pyrethroid-resistant Musca domestica at the molecular level and study its resistance-related mechanism better, this study was based on the results of P4 50 gene MDC YP6D1 conserved sequence Designed primers were synthesized by PCR from the house fly in Beijing also amplified a specific fragment of about 2101 bp fragment length similar to the original sequence corresponding fragment that Beijing The flies P450 gene has a great homology with MDCYP6D1. H inf I did not find the corresponding restriction sites on the amplified product, indicating that the amplification product is different from the corresponding part of the original sequence MDCYP6D1, suggesting that this PCR method amplified from the housefly in Beijing P450 gene fragment may correspond to a new P450 gene. The results of PCR-SSCP showed that there was some polymorphism in the primary structure of the amplified product, which may be related to the complex diversity of P450 gene in insects