用外植体一农杆菌共培养法将酵母脯氨酸合成酶基因2(pro 2)导入豆科牧草紫云英,获得转基因植株。Southern blot分析检测到转化植株基因组中存在外源DNA的同源顺序,证明酵母pro 2基因已整合到紫云英细胞基因组中。转化植株具有强的NPT Ⅱ酶活性,而对照植株呈阴性反应。在含0.5%NaCl的培养基上,紫云英植株内游离脯氨酸含量增高,一些转化植株叶片内游离脯氨酸含量显著高于对照,而且耐盐性有所提高。转化植株移栽到土壤中开花结实,收获到R_1代种子。 Using the explant-agrobacterium co-culture method, the yeast pro-2 synthase gene 2 (pro 2) was introduced into the legume pasture and the transgenic plants were obtained. Southern blot analysis detected the presence of exogenous DNA homologous sequences in the transformed plant genome, demonstrating that the yeast pro 2 gene has been integrated into the genome of the milk vetch. The transformed plants had strong NPT Ⅱ enzyme activity, whereas the control plants were negative. The content of free proline was increased in the medium containing 0.5% NaCl, the content of free proline in leaves of some transformed plants was significantly higher than that of the control, and the salt tolerance was improved. Transformed plants transplanted into the soil flowering and fruiting, R_1 generation of seeds harvested.