Background Choledochal cyst excision and biliary enteric reconstruction constitute the best therapy for choledochal cyst.And laparoscopy is currently used to cure this disease now.Methods We retrospectively analyzed the clinical data of 34 cases of total laparoscopic choledochal cyst excision between January 2007 and August 2011.All patients underwent in vitro Roux-en-Y hepatoenterostomy.Results All 34 patients underwent successful total laparoscopic choledochal cyst excision.The operation time was 200-360 minutes.The duration of hospital stay was 3-7 days.Follow-up observations lasted 1-56 months.One patient developed an anastomotic stoma stricture,but no other cases had postoperative complications.No patients died.Conclusion Total laparoscopic choledochal cyst excision is safe and feasible.