论文部分内容阅读
目的研究腹膜乳斑与胃癌细胞在腹膜播散中的关系 .方法 30只经环孢素 A处理的 SD大鼠分成 3组 ,分别向大鼠腹腔内接种经溴脱氧尿甙 (BrdU)标记的 Sy86B裸鼠可移植性人胃癌细胞 ,数量分别为 105、 106和 107.应用免疫组织化学染色 ,观察癌细胞的分布 .计数网膜乳斑或非乳斑区标记细胞的数量 ,确定癌细胞的分布 .结果在乳斑或非乳斑区平均标记细胞数的比率分别是 163∶ 1(105)、 280∶ 1(106)和 697∶ 1(107).标记细胞数在乳斑或非乳斑区之间的差异有显著性意义 (P< 0 01).结论标记细胞选择性地侵入网膜乳斑 ,表明在胃癌腹膜播散的早期 ,癌细胞具有特异地侵入腹膜乳斑的特性 .
Objective To study the relationship between peritoneal milk spot and gastric cancer cells in peritoneal dissemination. METHODS: Thirty SD rats treated with cyclosporine A were divided into 3 groups. Rats were intraperitoneally inoculated with BrdU labeled with bromodeoxyuridine (BrdU). Sy86B transplantable human gastric cancer cells in nude mice were counted as 105, 106, and 107, respectively. Immunohistochemical staining was used to observe the distribution of cancer cells. The number of labeled cells in omental or non-milky plaque was counted to determine the number of cancer cells. Distribution. Results The average number of labeled cells in the milky or non-milky plaque area was 163:1 (105), 280:1 (106), and 697:1 (107), respectively. The number of labeled cells in milk spots or non-blotchy plaques The difference between the two regions was significant (P<001). Conclusions Marker cells selectively invade the omental milk spot, indicating that in the early stage of gastric cancer peritoneal dissemination, cancer cells specifically invade the peritoneal milk spot.