Objective To discuss the regulating effect of Sargassum fusiforme (Harv. ) Setch. on the level of blood lipid in experimental rats with hyperlipidemia. Methods To establish animal models with hyperlipidemia,high fat forage was fed to 40 healthy female Wistar rats. Then they were fed S. fusiforme powder with the purpose of intervention treatment. Biochemical techniques were adopted to detect the levels of triglyceride (TG) ,total cholesterol (TC) ,low density lipoprotein (LDL) ,high density lipoprotein (HDL) ,and the activities of lipoprotein lipase (LPL) and hepatic lipase (HL) . Enzyme linked immunosorbent assay was used to measure the level of hydroxy methylglutaryl coenzyme A (HMG-CoA) and the activity of hydroxy methylglutaryl coenzyme A reductase (HMG-CR) . Results TG,TC and LDL levels of blood serum in treatment group were significantly lower than those in model group (P < 0. 05) ,but HDL level was significantly increased (P < 0. 05) . LPL and HL levels of blood serum and liver tissue were significantly higher than those in model group (P < 0. 05) ,HMG-CoA level was signif-icantly higher in liver tissue than in model group,but HMG-CR activity was significantly lower in liver tissue than in model group (P < 0. 05) . Conclusions S. fusiforme could affect the metabolism of blood fat components and regulate the level of blood fat via enhancement of LPL. HL and HMG-CR activities. On the level of blood lipid in experimental rats with hyperlipidemia, high fat forage was fed to 40 healthy female Wistar rats. Then they were fed S. fusiforme powder with the purpose of intervention treatment. Biochemical techniques were adopted to detect the levels of triglyceride (TG), total cholesterol (TC), low density lipoprotein (LDL), high density lipoprotein Enzyme linked immunosorbent assay was used to measure the level of hydroxy methylglutaryl coenzyme A (HMG-CoA) and the activity of hydroxy methylglutaryl coenzyme A reductase (HMG-CR). Results TG, TC and LDL levels of blood serum in treatment group were significantly lower than those in model group (P <0.05), but HDL level was significantly increased (P <0.05). LPL and HL levels of blood serum and liver t issue were significantly higher than those in model group (P <0.05), HMG-CoA level was signif-icantly higher in liver tissue than in model group, but HMG-CR activity was significantly lower in liver tissue than in model group ( P <0.05). Conclusions S. fusiforme could affect the metabolism of blood fat components and regulate the level of blood fat via enhancement of LPL. HL and HMG-CR activities.