论文部分内容阅读
本实验以大豆萌动种胚为受体，用含有３５Ｓ启动子—Ｇｕｓ基因—ｉｐｔ基因—Ｎｏｓ基因的融合基因根癌农杆菌ＬＢＡ４４０４进行转化。通过对转化及对照植株苗期子叶的过氧化物酶同工酶及酯酶同工酶分析，结果表明酶谱有明显差异，过氧化物酶同工酶有两条差异带：Ｒｆ０．５０９及Ｒｆ０．７１２；酯酶同工酶有一条差异带：Ｒｆ０．５３１。综合分析，上述三条差异带都显示的转化植株占所分析的转化植株的１４．４％。此法可作为转基因植株早期筛选的方法之一。
In this experiment, the germinating embryos of soybean were used as recipients and transformed with the Agrobacterium tumefaciens LBA4404 containing the 35S promoter-Gus gene-ipt gene-Nos gene. Through the analysis of peroxidase isoenzyme and esterase isozymes in seedling cotyledons of transformed and control plants, the results showed that there was a significant difference in the enzyme spectrum. Peroxidase isozymes had two different bands: Rf0.509 and Rf0.712; esterase isozyme has a difference with: Rf0.531. Taken together, the above three bands showed that the transformed plants accounted for 14.4% of the transformed plants analyzed. This method can be used as one of the early screening methods for transgenic plants.