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目的:探索谷氨酰胺对肿瘤生长及肝癌细胞凋亡的影响。方法:在小鼠右腋皮下接种H22肿瘤细胞悬液,灌服含谷氨酰胺(GLN)液;在人肝癌细胞培养液中加入不同浓度的GLN液。分别检测小鼠血浆及细胞培养液中MDA、NO及细胞中GSH含量,观察小鼠右腋皮下肿瘤生长及肝癌细胞增殖和凋亡情况。结果:灌服GLN液,有抑制皮下肿瘤块生长的作用;在人肝癌细胞培养液中加入一定浓度的GLN液,有抑制肝癌细胞增殖的作用并促使肝癌细胞凋亡。同时小鼠血浆和细胞培养液中NO含量升高,MDA稍有下降;细胞粉碎液中,GSH升高。结论:GLN对肿瘤生长的抑制作用及对肝癌细胞凋亡的影响可能与使抗氧化活性的提高、阻抑自由基对癌细胞增殖的介导及促使NO释放有关。
Objective: To explore the effect of glutamine on tumor growth and apoptosis of hepatoma cells. METHODS: H22 tumor cell suspension was inoculated subcutaneously into the right iliac crest of mice and fed with glutamine (GLN) solution. Different concentrations of GLN solution were added to the culture solution of human hepatoma cells. The levels of MDA, NO, and GSH in the cells were measured. The growth of right subcutaneous tumors and the proliferation and apoptosis of hepatoma cells were observed. Results: The GLN solution could inhibit the growth of subcutaneous tumor mass. Adding a certain concentration of GLN solution in the culture solution of human hepatoma cells could inhibit the proliferation of hepatoma cells and promote the apoptosis of hepatoma cells. At the same time, NO content in mouse plasma and cell culture fluids increased, MDA slightly decreased; GSH increased in cell comminuted fluid. Conclusion: The inhibitory effect of GLN on tumor growth and apoptosis in hepatoma cells may be related to the increase of antioxidative activity, inhibition of free radicals on the proliferation of cancer cells and promotion of NO release.