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目的:对虾夷扇贝生殖腺酶解液的凝胶特性进行研究,为虾夷扇贝的综合利用提供研究基础。方法:以雄性生殖腺为原料,采用中性蛋白酶进行酶解,用质构仪和流变仪测定酶解液的凝胶特性,并通过分析氨基酸组成,分子质量变化和脱氧核糖核酸酶(DNase)的作用探讨凝胶形成机理。结果:虾夷扇贝雄性生殖腺经中性蛋白酶(3 000 U/g蛋白)酶解,随着水解时间的延长,酶解液的硬度、凝聚性和黏附力均显著提高,在3 h时可分别达到(80.86±6.87)g,(600.98±91.05)g·s和(28.85±6.17)g。该酶解液在质量浓度50~100 mg/m L范围,储能模量G′占主导,显示出弹性特性。随着酶解液质量浓度的增大,G′、损耗模量G″和复数黏度η*均增大。酶解液具有明显的剪切稀化现象,为非牛顿流体中的塑料流体。虾夷扇贝雄性生殖腺中含有脯氨酸、亮氨酸、丙氨酸等疏水性氨基酸,酶解后高于14.3 ku的条带明显降解,通过DNase处理可明显降低酶解液的凝胶性。结论:虾夷扇贝雄性生殖腺酶解液的凝胶特性可能与疏水性氨基酸和脱氧核糖核酸有关。
OBJECTIVE: To study the gelation characteristics of zygotic xanthoceras enzymolysis solution and provide the basis for the comprehensive utilization of yacon scallop. Methods: The male gonad was used as raw material, neutral protease was used for enzymatic digestion. The gel characteristics of the hydrolyzate were determined by means of mass spectrometer and rheometer. The amino acid composition, molecular weight and DNAase The role of gel formation mechanism. Results: The male gonad was digested with neutral protease (3 000 U / g protein). With the prolongation of hydrolysis time, the hardness, cohesion and adhesion of the hydrolyzate increased significantly Reached (80.86 ± 6.87) g, (600.98 ± 91.05) g · s and (28.85 ± 6.17) g respectively. The hydrolyzate in the mass concentration of 50 ~ 100 mg / m L range, storage modulus G ’dominant, showing the elastic properties. With the increase of mass concentration of enzymatic hydrolyzate, G ’, loss modulus G "and complex viscosity η * increased.Enzymic hydrolyzate had obvious shear thinning phenomenon and was a plastic fluid in non-Newtonian fluid. The male gonads of Prunus scabi contained hydrophobic amino acids such as proline, leucine and alanine, and the bands higher than 14.3 ku were degraded obviously after DNase treatment, and the gelation of the hydrolyzate could be obviously reduced by DNase treatment. : The gel properties of zygote male reproductive zymogen solution may be related to hydrophobic amino acids and DNA.