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为了给肿瘤的生物学治疗提供形态学基础,试验分离和培养树突状细胞(DC),制备B16黑色素瘤细胞抗原并进行共培养,即为抗原负载树突状细胞(ADC);建立B16黑色素瘤小鼠模型,于瘤周围皮下注射DC和ADC,测量注射前后各组小鼠的瘤体积,比较其抑瘤作用;应用光镜和透射电镜观察DC和ADC诱导特异性CTL抗肿瘤效应的形态学表现。结果表明:试验组DC和ADC诱导机体抗肿瘤效应显著,与对照组相比差异显著(P<0.05);光镜下DC和ADC主要分布在皮下组织、癌组织周围,特别是癌巢周边;透射电镜下与ADC接触的淋巴细胞形态不规则,淋巴细胞与肿瘤细胞密切接触,大量肿瘤细胞凋亡、坏死。
In order to provide a morphological basis for the biological treatment of tumors, dendritic cells (DCs) were isolated and cultured experimentally to prepare B16 melanoma cell antigens for co-culture, ie, antigen-loaded dendritic cells (ADCs) Tumor mice model, DCs and ADCs were injected subcutaneously around the tumor, and the tumor volume of mice in each group was measured before and after injection to compare their anti-tumor effects. The morphological changes of CTLs induced by DCs and ADCs were observed by light microscope and transmission electron microscope Learning performance. The results showed that the anti - tumor effects of DC and ADC in the experimental group were significant (P <0.05), and the distribution of DC and ADC were mainly in the subcutaneous tissue and the surrounding of the cancer tissue, especially around the cancer nests. Under TEM, lymphocytes contacted with ADC were irregular in shape, lymphocytes were in close contact with tumor cells, and a large number of tumor cells were apoptosis and necrosis.