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目的:通过雷公藤多苷(TG)对特发性血小板减少性紫癜(ITP)小鼠的T细胞亚群的影响及疗效观察,探讨雷公藤多苷治疗ITP作用机制。方法:采用现代医学免疫法腹腔注射豚鼠抗小鼠血小板抗血清(APS),建立ITP动物模型。小鼠随机分为5组,每组12只,分别为正常组,模型组,TG大、小剂量组,泼尼松组,均从第1次注射APS36h后灌胃,每日1次,连续10d,实验结束后,检测外周血象、骨髓象,流式细胞术检测T淋巴细胞亚群。结果:雷公藤多苷大剂量组有明显回升血小板、降低骨髓巨核细胞、CD8+明显降低及CD4+/CD8+上升的作用。结论:大剂量雷公藤多苷对ITP模型小鼠有明显治疗作用,使造模后动物死亡率明显降低,血小板及血红蛋白明显上升白、骨髓巨核细胞数明显下降,T淋巴细胞亚群得以改善,提示其疗效可能是通过对细胞免疫功能的调节而发挥作用的。
OBJECTIVE: To investigate the effects of Tripterygium glycosides on the T cell subpopulation of mice with idiopathic thrombocytopenic purpura (ITP) and to observe the therapeutic effect. Methods: The animal model of ITP was established by intraperitoneal injection of guinea pig anti-mouse platelet antiserum (APS) using modern medical immunization. The mice were randomly divided into 5 groups of 12 in each group: normal group, model group, large and small doses of TG, and prednisone group. Each group received intragastric administration from the first injection of APS for 36 hours, once a day, continuously. After 10 days, peripheral blood and bone marrow were detected after the experiment. Flow cytometry was used to detect T lymphocyte subsets. RESULTS: High-dose glycosides of Tripterygium wilfordii significantly increased platelet counts, decreased bone marrow megakaryocytes, significantly decreased CD8+, and increased CD4+/CD8+ levels. Conclusion: High-dose tripterygium glycosides have obvious therapeutic effects on ITP model mice, and the animal mortality rate after model establishment is significantly reduced, platelet and hemoglobin are significantly increased, white and bone marrow megakaryocytes are significantly decreased, and T lymphocyte subsets are improved. It suggests that its efficacy may play a role by regulating the cellular immune function.