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世界人口持续增加,各种气候灾害频发,这两个因素给农业生产造成越来越严重的威胁。鉴定植物抗逆相关基因,利用基因工程和分子育种技术提高农作物对非生物胁迫的抗性是解决这个问题的主要途径。MYB转录因子家族是植物中广泛存在、具有重要功能的一类转录因子,在植物各种生理过程中起中心的调节作用,如调控植物特有的次级代谢过程或细胞壁形态过程,以及对生物和非生物胁迫的抗性。本研究利用生物信息学方法对蒺藜苜蓿全基因组中的MYB基因家族进行筛选,对筛选出的完整MYB基因结构、染色体定位、系统发生、基因表达特征等进行分析,并与天蓝苜蓿转录组测序获得的MYB基因家族进行比较,旨在为苜蓿植物育种提供重要的理论依据。我们在蒺藜苜蓿的基因组中鉴定出219个MYB基因。这些基因不均匀地分布在蒺藜苜蓿的8条染色体上,5号染色体最多(45个)。以拟南芥的MYB基因为outgroup,我们构建了蒺藜苜蓿、天蓝苜蓿与拟南芥的MYB基因系统发育图。利用已有的基因芯片表达数据,分析了蒺藜苜蓿MYB基因的表达特点,发现了一些响应盐胁迫、根腐病菌侵染和菌根真菌共生的MYB基因。本研究的研究结果有助于蒺藜苜蓿MYB基因家族的功能分析和挖掘的研究。
The continuous increase of the world population and the frequent occurrence of various climate disasters have posed a growing threat to agricultural production. Identification of plant stress related genes, the use of genetic engineering and molecular breeding techniques to improve crop resistance to abiotic stress is the main way to solve this problem. MYB transcription factor family is a kind of transcription factor widely distributed in plants, which has important functions. It plays a central regulatory role in various physiological processes of plants, such as the regulation of plant-specific secondary metabolic processes or cell wall morphological processes, Abiotic stress resistance. In this study, the MYB gene family of Medicago truncatula screening was screened by bioinformatics methods, and the complete MYB gene structure, chromosomal location, phylogeny and gene expression characteristics were screened, and compared with the MYB gene family of Medicago truncatula MYB gene family comparison, aimed at alfalfa plant breeding provides an important theoretical basis. We identified 219 MYB genes in the Medicago truncatula genome. These genes are unevenly distributed on the 8 chromosomes of Medicago truncatula, with the largest number on chromosome 5 (45). To MYB gene of Arabidopsis outgroup, we constructed the Medicago truncatula, Alfalfa and Arabidopsis MYB gene phylogenetic map. According to the existing expression data of gene chip, the expression characteristics of MYB gene in Medicago truncatula were analyzed, and some MYB genes were found to respond to salt stress, root rot pathogen infection and mycorrhizal fungi. The results of this study contribute to the functional analysis and mining of the MYB gene family of Medicago truncatula.