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目的探讨人脐血间充质干细胞(h UCB-MSCs)移植对大鼠创伤性脑损伤(TBI)模型炎性细胞分泌的调节及脑保护作用机制。方法将用Brdu标记的h UCB-MSCs经鼠尾静脉植入大鼠TBI模型。检测和比较假伤组、损伤组和移植组大鼠血清细胞黏附分子-1(ICAM-1)含量、白细胞计数(WBC)及多形核嗜中性白细胞(PMN)计数及脑组织髓过氧化物酶(MPO)活性的改变。结果与假伤组相比,TBI模型大鼠血ICAM-1、WBC及PMN计数、脑组织MPO活性明显增加。h UCB-MSCs移植后各时间点上述指标表达较损伤组减低;至注射后7 d,移植组血ICAM-1、WBC及PMN计数已接近假伤组水平,脑组织MPO表达仍高于假伤组水平(P<0.05)。经相关性分析,s ICAM-1与外周血PMN计数、白细胞计数与PMN计数、PMN计数与MPO活性表达均呈正相关。结论经尾静脉注射h UCB-MSCs能有效促进脑组织损伤的修复,其机制可能与减少血ICAM-1、WBC及PMN的数量、抑制脑组织MPO的分泌有关。
Objective To investigate the effects of hUCB-MSCs transplanted on the inflammatory cytokines secretion and brain protective mechanism of traumatic brain injury (TBI) in rats. Methods Brdu-labeled h UCB-MSCs were implanted into the rat model of TBI via tail vein. The levels of ICAM-1, WBC, PMN count and the levels of myeloperoxidase in brain tissue of sham injury group, injury group and transplantation group were detected and compared. Changes in enzyme activity (MPO) activity. Results Compared with the sham injury group, the ICAM-1, WBC and PMN counts in blood of TBI model rats significantly increased MPO activity in brain tissue. h after transplantation of UCB-MSCs, the expression of ICAM-1, WBC and PMN in the transplantation group was close to that of the sham-injury group, and the level of MPO in the brain tissue was still higher than that of the sham-injury group Group level (P <0.05). Correlation analysis showed that there was a positive correlation between s ICAM-1 and peripheral blood PMN count, leucocyte count and PMN count, PMN count and MPO activity. Conclusion The hUCB-MSCs injected through the caudal vein can effectively promote the repair of brain injury. The mechanism may be related to reducing the number of ICAM-1, WBC and PMN, and inhibiting the secretion of MPO.