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本研究探讨NOV及BNIP3基因在小鼠粒单核细胞白血病中的表达及意义。给小鼠经尾静脉接种小鼠粒单核细胞白血病细胞株WEHI-3,随机将小鼠分成化疗组和对照组,然后在接种后不同时间点取2组小鼠骨髓组织,采用qRT-PCR方法动态检测NOV、BNIP3 mRNA的表达情况,分析其表达与白血病发生及发展的关系。结果表明:对照组小鼠NOV、BNIP3 mRNA表达水平在接种后2周开始逐渐增高,死亡时达到高峰,分别由接种前的1.85E-05与3.44E-03,升高至濒死时的3.57E-02与3.48E-02(p<0.05)。化疗组小鼠2种基因的表达率则在化疗后迅速降至2.51E-05与1.58E-03(p<0.05),与接种前水平相近(p>0.05),复发时再次升高,死亡时2基因表达率与对照组无明显差异(p>0.05)。结论:NOV及BNIP3基因在白血病小鼠中表达明显增高,其表达异常可能参与了小鼠白血病的发生与发展;2种基因表达率与疗效密切相关,因此NOV和BNIP3表达水平在疗效评估及MRD检测中具有重要价值。
This study was to investigate the expression and significance of NOV and BNIP3 in mouse myelomonocytic leukemia. Mice were inoculated with WEHI-3 into the tail vein to divide the mice into chemotherapy group and control group. Then, two groups of mice bone marrow tissues were taken at different time points after inoculation, and the mice were treated with qRT-PCR Methods The expression of NOIP and BNIP3 mRNA was detected dynamically, and the relationship between the expression of BNIP3 mRNA and the occurrence and development of leukemia was analyzed. The results showed that the expression levels of NOIP and BNIP3 mRNA in control mice began to increase at 2 weeks after inoculation, reaching the peak at the time of death, from 1.85E-05 and 3.44E-03 before inoculation to 3.57 E-02 and 3.48E-02 (p <0.05). After chemotherapy, the expression rates of the two genes in the chemotherapy group decreased rapidly to 2.51E-05 and 1.58E-03 (p <0.05), which were similar to those before inoculation (p> 0.05) When 2 gene expression rate and the control group no significant difference (p> 0.05). CONCLUSION: The expression of NOV and BNIP3 in leukemia mice was significantly higher than that in normal mice. The abnormal expression of NOV and BNIP3 may be involved in the occurrence and development of mouse leukemia. The expressions of NOV and BNIP3 are closely related to the therapeutic effect. Detection of great value.