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目的 探索血管紧张素转换酶(angiotensionconvertingenzyme,ACE)基因多态性与冠心病的关联分析。方法 应用聚合酶链反应(PolymeraseChainReaction,PCR)技术对 100例住院冠心病患者及 90例正常对照者进行ACE基因多态性测定,并对冠心病患者进行A型行为类型问卷(TypeABehaviorPatternScale,TABP)测定。结果 冠心病组的DD基因型频率 (34% )及D等位基因频率 (66% )均显著高于正常对照组(14. 4%, 44. 4% ),且RRDD=3. 05,RRD=2. 43,P<0. 05,表明冠心病与ACE基因的DD基因型、D等位基因呈显著正关联;具有A型行为的冠心病患者的DD基因型频率及D等位基因频率均显著高于非A型患者(P<0. 05);携带DD基因型的冠心病患者的A型行为问卷的TH因子 (15. 88±3. 16)、CH因子分(14. 88±2. 89)极显著地高于携带ID及Ⅱ基因型患者的因子分 ( 12. 89±4. 53, 12. 52±3. 88, 13. 00±1. 41, 10. 50±0. 71) (P<0. 01)。结论 ACE基因多态性可能是冠心病发生发展过程中重要的危险因素之一,其DD型和D等位基因与冠心病患者的A型行为有一定的联系。
Objective To explore the association between angiotensin converting enzyme (ACE) gene polymorphism and coronary heart disease. Methods Polymerase chain reaction (PCR) was used to detect ACE gene polymorphism in 100 hospitalized coronary heart disease patients and 90 healthy controls, and Type A Behavior Pattern Scale (TABP) was measured in patients with coronary heart disease. . Results The frequency of DD genotype (34%) and D allele (66%) in CHD group were significantly higher than those in control group (14.4%, 44.4%), and the RRDD was 3.55 = 2.43, P <0.05, indicating that coronary heart disease and ACE gene DD genotypes, D allele was significantly correlated; type A behavior in patients with coronary heart disease DD genotype frequency and D allele frequency (P <0.05). The A-type behavioral questionnaire of CHD patients with DD genotype had significantly higher TH (15.88 ± 3.16) and CH (14.88 ± 2.89) were significantly higher than those in patients with ID and type II genotypes (12.89 ± 4.53, 12.52 ± 3.88, 13.00 ± 1.41, 10.50 ± 0. 71) (P <0.01). Conclusion ACE gene polymorphism may be one of the important risk factors in the development of coronary heart disease. The DD type and D allele have a certain relationship with type A behavior in patients with coronary heart disease.