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目的 确定晶状体谷胱甘肽 (GSH)转运子的特性 ,检测白内障晶状体内该转运子的调节功能。 方法 有关GSH转运的研究方法如下 :( 1)原位眼部血管灌注 ;( 2 )非洲爪蛙卵注射 ;( 3 )人晶状体上皮细胞培养。 结果 研究表明 ,晶状体对GSH比对蔗糖的摄取要高几倍 ,同时还发现晶状体上皮比皮质摄取的速度要快得多。晶状体poly(A) + RNA蛙卵注射对GSH的摄取表达结果表明 ,晶状体上皮仅有一部分对Na+ 具有依赖性。摄取显示出晶状体上皮对Na+ 具有高亲和性和低亲和性两个转运系统。动物在喂养半乳糖 1天后 ,可明显降低晶状体对GSH的摄取。 结论 在哺乳动物晶状体内 ,存在有依赖Na+ 和非依赖Na+ 的GSH转运。半乳糖性白内障GSH转运能力的降低部分原因可能与较低的晶状体GSH水平有关
Objective To determine the characteristics of lens glutathione (GSH) transporter and to examine the regulatory function of this transporter in cataractous lens. Methods Methods of GSH transport were as follows: (1) in situ ocular perfusion; (2) Xenopus injection; (3) human lens epithelial cell culture. Results Studies have shown that lens uptake of GSH is several times higher than that of sucrose and that the lens epithelium is much faster than the cortex. The uptake of GSH by the lens poly (A) + RNA frog egg injection showed that only a part of the lens epithelium was Na + -dependent. Uptake showed two transport systems with high affinity and low affinity for the lens epithelium. Animals fed galactose for 1 day significantly reduced the lens’s uptake of GSH. Conclusion In mammalian lens, there are GSH transport dependent on Na + and Na + independent. Part of the reason for the decreased GSH transport capacity of galactose cataract may be related to lower lens GSH levels