Multilayer film was fabricated on an electrode surface by alternate layer-by-layer(LBL) adsorption of polycationic redox polymer(PEI-Fc) and dye-linked L-proline dehydrogenase(L-proDH).The electrochemistry of the PEI-Fc/L-proDH multilayer modified electrode was investigated by cyclic voltammetry,and the enzyme catalysis mediated by the redox polymer was studied in a solution containing L-proline.It was observed that electron communication between L-proDH and the electrode was achieved with the help of PEI-Fc.A mathematical expression for the current response was evaluated based on the Michaelis-Menten kinetics mode,and the calculated currents fitted well with the experimental data.The kinetic analysis indicates that only a small fraction of the immobilized enzyme was efficiently electrically wired by the redox polymer. Multilayer film was fabricated on an electrode surface by alternate layer-by-layer (LBL) adsorption of polycationic redox polymer (PEI-Fc) and dye- linked L-proline dehydrogenase (L-proDH). The electrochemistry of the PEI-Fc / L-proDH multilayer modified electrode was investigated by cyclic voltammetry, and the enzyme catalysis mediated by the redox polymer was studied in a solution containing L- proline. It was observed that electron communication between L-proDH and the electrode was achieved with the help of PEI-Fc. A mathematical expression for the current response was evaluated based on the Michaelis-Menten kinetics mode, and the calculated currents fitted well with the experimental data. Kinetic analysis indicates that only a small fraction of the immobilized enzyme was efficiently electrically wired by the redox polymer.