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目的:研究炎症性肠病(IBD)患者外周血T细胞受体删除DNA环(TRECs)的含量,了解IBD患者胸腺近期输出功能变化,探讨其在IBD发病中的意义。方法:采用实时荧光定量聚合酶链反应(realtime PCR)检测20名健康人、15例活动期克罗恩病(CD)患者和22例活动期溃疡性结肠炎(UC)患者外周血T细胞中TRECs的含量。结果:TRECs含量CD患者13.48±10.59、UC患者14.67±11.12,各组患者每1000个外周血T细胞中TRECs含量均明显高于健康组8.32±7.02(P均<0.05)。结论:IBD患者幼稚T细胞的含量显著高于正常人,提示IBD患者胸腺近期输出功能在炎症活动期明显增强。
OBJECTIVE: To study the content of T cell receptor-deleted DNA loops (TRECs) in peripheral blood of patients with inflammatory bowel disease (IBD) and to investigate the changes of short-term output function of thymus in IBD patients and its significance in the pathogenesis of IBD. Methods: Real-time quantitative PCR (realtime PCR) was used to detect T cells in 20 healthy volunteers, 15 patients with active Crohn’s disease (CD) and 22 patients with active ulcerative colitis (UC) TRECs content. Results: The levels of TRECs in TRECs were 13.48 ± 10.59 in UC patients and 14.67 ± 11.12 in UC patients, respectively. The levels of TRECs per 1000 peripheral blood T cells in each group were significantly higher than those in healthy subjects (8.32 ± 7.02, P <0.05). Conclusion: The content of naive T cells in IBD patients is significantly higher than that in normal subjects, suggesting that the short-term thymus output in IBD patients is significantly increased during the inflammatory phase.