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A new method was developed for simultaneous determination of levels of AMP,ADP and ATP in blood by anion-exchange chromatography.The three adenosine phosphates were separated by Dionex IonPac AG18(50 mm×4 mm) guard column and IonPac AS 18(250 mm×4 mm) analytical column using a gradient method and detected with a suppressed conductivity detector. The detection limits(S/N=3) of AMP,ADP and ATP were 38,47,108 ng/L,respectively.The relative standard deviations of retention time,peak area and peak height were all less than 1.87%and a good linear relationship was obtained.This method was applied to analyze human blood samples.
A new method was developed for simultaneous determination of levels of AMP, ADP and ATP in blood by anion-exchange chromatography. The three adenosine phosphates were separated by Dionex IonPac AG18 (50 mm × 4 mm) guard column and IonPac AS 18 (250 mm The detection limits (S / N = 3) of AMP, ADP and ATP were 38, 47, 108 ng / L, respectively. The relative standard deviations of retention time, peak area and peak height were all less than 1.87% and a good linear relationship was.This method was applied to analyze human blood samples.