论文部分内容阅读
本文对２０例乳腺浸润性导管癌，２５例乳腺纤维腺瘤，３０例乳腺小叶增生的病理组织细胞进行图像分析，主要定量测定核仁组成区嗜银蛋白（ＡｇＮＯＲｓ）颗粒的数目、面积和核面积；再利用Ｐ５３、Ｃ－Ｎｅｕ单抗进行组织细胞的免疫组化测定，同时用ＥＬＩＳＡ方法测定患者外周血清层粘蛋白（ｌａｍｉｎｉｎ，ＬＮ）。结果发现：在病变细胞内，ＡｇＮＯＲｓ颗粒面积和核面积的比值不但在各病变组与正常乳腺组织有显著性差异（Ｐ＜０．０５），而且与Ｐ５３、Ｃ－Ｎｅｕ之间也有一定相关性，随着肿瘤的恶化和浸润程度的改变，细胞外间质物质之一的层粘蛋白的含量也会有一定范围的变化，提示肿瘤细胞内蛋白异常增殖，ＡｇＮＯＲｓ颗粒变化不但与细胞基因调控失常有关，也直接影响细胞外间质ＬＮ的含量变化，使得血清中ＬＮ含量增高。从而发现细胞内ＡｇＮＯＲｓ颗粒数和血清ＬＮ二者之间存在一定的相关性（ｒ＝０．９２）。这将为乳腺细胞的病变、恶化、浸润的实验诊断提供了一定的依据。
In this paper, 20 cases of invasive ductal carcinoma of the breast, 25 cases of breast fibroadenoma, and 30 cases of lobular hyperplasia of the breast tissue were analyzed by image analysis, mainly to quantitatively determine the number, area and nucleus of AgNORs in nucleolar organizer regions Area. The immunohistochemistry of tissue cells was performed using P53 and C-Neu monoclonal antibodies. Serum laminin (LN) was also measured by ELISA. The results showed that in the diseased cells, the ratio of AgNORs particle area to the nucleus area was not only significantly different from normal breast tissues (P <0.05), but also with P53 and C-Neu , As the tumor progresses and the degree of infiltration changes, the level of laminin in the extracellular matrix substance will also change to some extent, suggesting abnormal proliferation of tumor cells. AgNORs particles not only have abnormal regulation of cell genes, Related, but also directly affect the changes in the content of extracellular matrix LN, making the serum increased LN content. It was found that there was a certain correlation between intracellular AgNORs particles and serum LN (r = 0.92). This will provide a basis for the experimental diagnosis of breast cell lesions, deterioration, infiltration.