论文部分内容阅读
目的建立人尿中N-甲基甲酰胺和N-甲基乙酰胺的气相色谱测定法,并应用该方法对某制革企业从业人员和周边居民尿中N-甲基甲酰胺和N-甲基乙酰胺含量进行检测,探讨N,N-二甲基甲酰胺和N,N-二甲基乙酰胺对人体健康的影响。方法采集待测人员尿液经离心分离、沉淀杂质后用FFAP毛细管色谱柱分离,串联FID检测器测定。同时检测待测人员血清中谷丙转氨酶含量,观察肝功能情况。结果制革企业内部接触人员尿液中检测出N-甲基甲酰胺和N-甲基乙酰胺,且谷丙转氨酶含量异常,周边居民均正常。从业人员尿中N-甲基甲酰胺和N-甲基乙酰胺的浓度并不随其工作年限的增长而增大,但与工作岗位有关。尿中N-甲基甲酰胺和N-甲基乙酰胺比谷丙转氨酶能更灵敏地反映人体肝功能情况,可作为评价N,N-二甲基甲酰胺和N,N-二甲基乙酰胺暴露水平的生物指标。结论 N,N-二甲基甲酰胺和N,N-二甲基乙酰胺对从业人员肝功能有损害,建议进行日常尿液监测。
Objective To establish a gas chromatographic method for the determination of N-methylformamide and N-methylacetamide in human urine and to apply the method to the urine of N-methyl formamide and N- Acetamide content were tested to explore the impact of N, N-dimethylformamide and N, N-dimethylacetamide on human health. Methods Urine collected from the test persons was separated by centrifugation and precipitated impurities were separated by FFAP capillary column and measured by serial FID detector. At the same time test serum alanine aminotransferase in the test, observe the liver function. Results In the urine of tannery workers, N-methylformamide and N-methylacetamide were detected in urine, and the content of alanine aminotransferase was abnormal, and the surrounding residents were normal. Concentrations of N-methylformamide and N-methylacetamide in the urine of practitioners did not increase with their working years, but were related to jobs. Urinary N-methyl formamide and N-methyl acetamide than the alanine aminotransferase can reflect more sensitive human liver function, can be used as a valuation of N, N-dimethylformamide and N, N- Biological indicators of amide exposure levels. Conclusion N, N-dimethylformamide and N, N-dimethylacetamide have impaired liver function in practitioners, and daily urine monitoring is recommended.