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目的通过对2015年昆明市东川区突发皮肤炭疽疫情的处理和实验室检测,提供处置此类事件的一种科学准确的方法和依据,以提供借鉴。方法用WS 283—2008炭疽诊断标准进行病原菌分离培养,用real-time PCR方法进行炭疽芽胞杆菌染色体编码的rpo B基因及质粒pXO1上pag A基因、pXO2上cap基因的检测及单核苷酸多态性(SNP)测定,用毛细管电泳进行多位点可变数目串联重复序列分析(MLVA)。结果从4例疑似皮肤炭疽患者的疱疹液中分离得到2株炭疽芽胞杆菌,且此2株菌的rpo B基因、pagA基因和cap基因均为阳性,对照分类表为A.Br.001/002亚群。结论此次皮肤炭疽疫情是由当地村民宰杀携带炭疽芽胞杆菌强毒株的病牛引起,与云南地区曾经存在的炭疽芽胞杆菌类型一致。
Objective To provide a scientific and accurate method and basis for the handling of such incidents by handling and laboratory testing of the outbreak of skin anthrax in Dongchuan, Kunming in 2015 to provide reference. Methods The pathogen was isolated and identified by the WS 283-2008 diagnostic criteria of anthrax. Real-time PCR was used to detect the rpo B gene encoded by B. anthracis and the pag A gene of pXO1. The detection of cap gene on pXO2 and single nucleotide (SNP) assay, and multi-site variable number tandem repeat analysis (MLVA) was performed by capillary electrophoresis. Results Two strains of Bacillus anthracis were isolated from the herpes fluid of four patients with suspected skin anthrax, and the two strains were positive for rpo B gene, pagA gene and cap gene. The control classification table was A.Br.001 / 002 Subgroups. Conclusion The skin anthrax epidemic was caused by the local villagers slaughtering the sick cattle carrying the strong strain of Bacillus anthracis, which was consistent with the type of Bacillus anthracis that existed in Yunnan before.