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研究了SP825大孔吸附树脂对款冬花总黄酮的吸附和解吸特性,并考察了相关的工艺条件。结果表明,最佳纯化工艺条件为原药液(总黄酮质量浓度为29.09 mg/mL)稀释5倍,调pH为3～4以2 BV/h的流速进行吸附,最大上样量为44倍树脂体积,3 BV的70%乙醇溶液以1～2 BV/h流速进行洗脱。经SP825大孔吸附树脂纯化后的款冬花总黄酮纯度达60%以上,具有较好的纯化效果。
The adsorption and desorption properties of SP825 macroporous resin on the total flavonoids of Coltsfoot were studied, and the related process conditions were investigated. The results showed that the optimum conditions for the purification of the original liquid (total flavonoids mass concentration of 29.09 mg / mL) diluted 5 times, adjusted to pH 3-4 flow rate of 2 BV / h adsorption, the maximum sample was 44 times Resin volume, 3 BV of 70% ethanol solution at 1 ~ 2 BV / h flow rate of elution. Purified by SP825 macroporous resin after purification of the total flavonoids of Coltsfoot flowers of more than 60%, with better purification.