目的　探索三氧化二砷 (As2 O3)联合酪氨酸激酶抑制剂木黄酮抑制慢性粒细胞性白血病 (CML)细胞增殖的效应及可能机制 ,为临床应用提供理论依据。方法　以CML细胞系K5 6 2为模型 ,通过细胞增殖检测、形态学观察、肿瘤集落形成和琼脂糖凝胶电泳手段 ,观察比较As2 O3 与木黄酮对CML细胞的增殖抑制效应。结果　经≥ 2 5 μmol/LAs2 O3 和 5mg/L木黄酮处理 2天后的K5 6 2细胞可出现增殖受抑和凋亡的形态学改变及DNA片段化 ,二者具有协同抑制作用。结论　As2 O3 与木黄酮能分别或协同抑制K5 6 2细胞生长 ,机制与诱导细胞凋亡有关 Objective To explore the effect of arsenic trioxide (As2O3) combined with tyrosine kinase inhibitor lignin on proliferation of chronic myelogenous leukemia (CML) cells and its possible mechanism, and provide theoretical basis for clinical application. Methods The cell proliferation assay, morphological observation, tumor colony formation and agarose gel electrophoresis were used to observe the effect of As2O3 and flavonoids on the proliferation of CML cells. Results K562 cells treated with ≥ 2 5 μmol/LAs2 O3 and 5 mg/L flavonoids could undergo morphological changes of apoptosis inhibition and apoptosis and DNA fragmentation, which had a synergistic inhibitory effect. Conclusion As2O3 and lignin can inhibit the growth of K562 cells respectively or synergistically, and the mechanism is related to the induction of apoptosis.