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以80份杂交籼稻亲本和部分新育成品系为试验材料,采用24对籼粳特异性分子标记对其进行材料间遗传多态性的划分,计算各材料间遗传距离,并以此为依据,通过UPGMA法进行相关聚类分析。结果表明:按遗传距离值的大小可将试验材料划分为5个大群、12个亚群和28个组群,大面积生产上主要应用的亲本80%以上(保持系、恢复系)均聚集在第Ⅰ大群内,分类结果和系谱分析基本吻合。结合杂交稻育种实践,群间优势普遍大于群内优势,表明利用籼粳特异分子标记在一定程度上区分杂交亲本优势群可行。
Twenty-four indica and japonica-specific molecular markers were used to segregate genetic polymorphism among 80 indica hybrids and some newly-cultivated hybrids, and the genetic distances among the materials were calculated. Based on this, UPGMA method for cluster analysis. The results showed that the test materials could be divided into five large groups, 12 subgroups and 28 groups according to the genetic distance value. Over 80% of the main parents (maintainer lines and restorers) of large-scale production were clustered in The first large group, the classification results and pedigree analysis basically consistent. Combining the practice of hybrid rice breeding, the superiority among the groups was generally greater than that within the group, indicating that the use of indica-japonica-specific molecular markers is feasible to distinguish the dominant groups of hybrid parents to a certain extent.