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  5. The Study on the Cellular and Molecular Mechanisms of Low intensity Pulsed Ultrasound in Human Prima

The Study on the Cellular and Molecular Mechanisms of Low intensity Pulsed Ultrasound in Human Prima

来源 :2005 World DNA and Genome Day(2005中国(大连)国际DNA和基因组节大会) | 被引量 : 0次 | 上传用户：zzggwd
【摘 要】
：
Objective:To explore the molecular mechanisms of the effect of low intensity pulsed ultrasound (US) on macrophages functions,and provide the theoretical foundation for its clinical application.Methods
【作 者】
：
Yiping Li Shaoxia Zhou Andreas Schmelz Max G.Bachem 
【机 构】
：
department of pathology, School of basic medical science, Southeast University, Nanjing, P.R.China,
【出 处】
：
2005 World DNA and Genome Day(2005中国(大连)国际DNA和基因组节大会)
【发表日期】
：
2005年12期
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　　Objective:To explore the molecular mechanisms of the effect of low intensity pulsed ultrasound (US) on macrophages functions,and provide the theoretical foundation for its clinical application.Methods:Ficoll-Paque density gradient centrifugation was used to get human blood macrophages,to analysis macrophages phagocytosis using fluorescein isothiocyanate (FITC)-labelled Escherichia coli (E.Coli),observe focal complexes and extracellular matrix metalloproteinases inducer (EMMPRIN) by fluorescence microscopy,to examine the secretion of metalloproteinases (MMPs) by gelatin zymography,and study the expression of Src,extracellular signal-regulated kinases (ERKs),and p38 mitogen activated protein kinase (MAPK) by Western blotting analysis.
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