【摘 要】
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Addressable and programmable DNA nanostructures are promising biomaterials to arrange multiple functional components for efficient and site-specific drug delivery.[1] Herein,we report a double bundle
【机 构】
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CAS Key Laboratory of Nanosystem and Hierarchical Fabrication,CAS Center for Excellence in Nanoscien
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Addressable and programmable DNA nanostructures are promising biomaterials to arrange multiple functional components for efficient and site-specific drug delivery.[1] Herein,we report a double bundle DNA tetrahedron(invented by Mao et al [2])rationally designed for delivery of antisense oligonucleotides silencing proto-oncogene c-raf.The antisense strands are located at the inside surface of the DNA tetrahedron to decrease the access of DNA digestive enzymes.Functionalized with nuclear targeting peptides,the DNA tetrahedron can be internalized by A549 cells and assists the delivery of antisense oligonucleotides towards the nucleus to increase the chance to down-regulate target mRNA in nucleus and cytoplasm.Antisense strands released from the tetrahedron in response to the intracellular reducing environment can inhibit cell proliferation at a low concentration without transfection reagent.Finally,efficient knockdown of c-raf gene is observed,which verified our design.This designed DNA-based nanocarrier system will open a new avenue for efficient delivery of nucleic acid drugs.[3]
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