【摘 要】
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(Objective)This experiment was conducted to investigate Trichomonas spp.infection in pigeons of Shandong Province.(Result)Oropharyngeal swabs(n =609)were collected randomly from 80,000 domestic pigeon
【机 构】
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Department of Preventive Veterinary Medicine,College of Veterinary Medicine,Shandong Agricultural Un
【出 处】
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中国畜牧兽医学会兽医寄生虫学分会第十三次学术研讨会
论文部分内容阅读
(Objective)This experiment was conducted to investigate Trichomonas spp.infection in pigeons of Shandong Province.(Result)Oropharyngeal swabs(n =609)were collected randomly from 80,000 domestic pigeons(Columba livia domestica)on five pigeon farms and at one pigeon slaughterhouse in Shandong Province,China,from September 2012 to July 2013.Trichomonas spp.were detected in 206/609(33.8%)samples.The prevalence was 14.9-31.1%,depending on different levels of sanitation and management,and was 4.8%in nestling pigeons,13.6%in breeding pigeons and 35.2%in adolescent pigeons.Trichomonas gallinae genotypes A and B,and Trichomonas tenax-like isolates,were identified by PCR-restriction fragment length polymorphism(RFLP)analysis and sequencing of the 5.8S rDNA-intemal transcribed spacer(ITS)regions.RFLP analysis with the restriction enzyme BsiEI generated different RFLP band pattems between T.gallinae and T.tenax-like isolates.(Conclusion)Trichomonas spp.infection in pigeons was common of Shandong Province.When BsiEI RFLP analysis was combined with HaeⅢ RFLP analysis,all infection types of T.gallinae and T.tenax-like isolates could be identified.
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