【摘 要】
：
(Objective)This experiment was conducted to investigate Trichomonas spp.infection in pigeons of Shandong Province.(Result)Oropharyngeal swabs(n =609)were collected randomly from 80,000 domestic pigeon
【机 构】
：
Department of Preventive Veterinary Medicine,College of Veterinary Medicine,Shandong Agricultural Un
【出 处】
：
中国畜牧兽医学会兽医寄生虫学分会第十三次学术研讨会
论文部分内容阅读
(Objective)This experiment was conducted to investigate Trichomonas spp.infection in pigeons of Shandong Province.(Result)Oropharyngeal swabs(n =609)were collected randomly from 80,000 domestic pigeons(Columba livia domestica)on five pigeon farms and at one pigeon slaughterhouse in Shandong Province,China,from September 2012 to July 2013.Trichomonas spp.were detected in 206/609(33.8%)samples.The prevalence was 14.9-31.1%,depending on different levels of sanitation and management,and was 4.8%in nestling pigeons,13.6%in breeding pigeons and 35.2%in adolescent pigeons.Trichomonas gallinae genotypes A and B,and Trichomonas tenax-like isolates,were identified by PCR-restriction fragment length polymorphism(RFLP)analysis and sequencing of the 5.8S rDNA-intemal transcribed spacer(ITS)regions.RFLP analysis with the restriction enzyme BsiEI generated different RFLP band pattems between T.gallinae and T.tenax-like isolates.(Conclusion)Trichomonas spp.infection in pigeons was common of Shandong Province.When BsiEI RFLP analysis was combined with HaeⅢ RFLP analysis,all infection types of T.gallinae and T.tenax-like isolates could be identified.
其他文献
Surveys of Cryptosporidium and Giardia duodenalis have rarely been performed in cats in China,despite their significance for public health.In this study,188 fecal samples were collected from five pet
Background Cryptosporidium spp.,Giardia duodenalis,and Enterocytozoon bieneusi are common gastrointestinal protists in humans and animals.Little information is available about these parasites in capti
Cryptosporidium parvum is the most important common enteropathogen inhabiting in many animals.Infections of C.parvum in immunocompetent livestock are commonly asymptomatic or self-limited,but it can b
根据GenBank上发表的贝氏贝诺孢子虫核糖体内转录间隔区(ITS)序列,在传统PCR方法的基础上设计一对特异性内引物,建立贝氏贝诺孢子虫巢氏PCR检测方法并进行了临床样品检测.结果表明,建立的巢式PCR检测方法能检测到贝氏贝诺孢子虫DNA的最低浓度为24.3 ag/μL,检测刚地弓形虫、犬新孢子虫、牛巴贝斯虫、环形泰勒虫、边缘无浆体等病原体均为阴性,196份临床检测样本发现,3份为阳性,阳性率为
This study investigated 319 fecal specimens of cats(n =52)and dogs(n =267)from Heilongjiang province,China for the prevalence and genetic characteristics of Cryptosporidium,Enterocytozoon bieneusi,and
Cryptosporidiosis,giardiasis and mierosporidiosis,three emerging infectious diseases caused by Cryptosporidium,Giardia and microsporidia in humans and animals,have been reported both in developed coun
为调查和研究贾第虫的流行性和基因型,使用SSU rRNA,tpi,gdh,和bg基因多位点分析方法,对采集的43个种类1,882份非人灵长类的粪便样品进行检测盒分析.结果显示,基于tpi基因,22个种类的122份(6.5％)非人灵长类的样本检测到贾第虫,包括恒河猴(2.7％),阿萨姆邦猕猴(11.1％),食蟹猴(10.0％),长尾猕猴(52.9％),日本猕猴(33.3％),白头叶猴(0.7％),赤
Giardia duodenalis is a common and widespread intestinal protozoan parasite of both humans and animals.Previous epidemiological and molecular studies have identified Giardia infections in different an
Giardia duodenalis and Cryptosporidium spp.are two of the most frequently identified gastro-intestinal protozoa causing diarrhea in cattle,particularly calves.Both protozoa are also considered as a po
Background: Giardia duodenalis is one of the most common enteric parasites in humans and animals,including companion animals,livestock and wildlife.To date,the information about the prevalence and mol