D-Peptides,as the enantiomers of the naturally occurring L-peptides,usually resist endogenous proteases and are presumably insensitive to most enzymes.But,it is unclear whether or how a phosphatase catalyzes the dephosphorylation from D-peptides.In this talk,we discuss the formation of the nanofibers of D-peptides via enzymatic dephosphorylation.By comparing the enzymatic hydrogelation of L-peptide and D-peptide based hydrogelators,we realized that the chirality of the precursors of the hydrogelators affects little on the enzymatic hydrogelation resulted from the removal of the phosphate group from a tyrosine phosphate residue.