Transformation of peanut with a 16D10 RNAi construct

来源 :The Sixth International Conference of the Peanut Research Co | 被引量 : 0次 | 上传用户:abc890619
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Root-knot nematodes (RKN) pose a great threat to peanut production in China and elsewhere.Earlier reports indicated the potential of inhibiting the nematodal 16D10 gene in management of the diseases.In this study, an artificially synthesized 16D10 RNAi structure with a gus intron was ligated into the multiple cloning site of pCAMBIA2300-OCS, driven by a CaMV35S promoter.The plant expression vector thus constructed was then transformed into Agrobacterium tumefaciens strain EHA105.
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