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利用犬心肌缺血再灌注模型观察纳络酮对缺血再灌注心肌腺苷酸环化酶（ＡＣ）活性和环磷酸腺苷（ｃＡＭＰ）含量的影响以及纳络酮在体外对心肌ＡＣ活性的影响。结果表明：心肌缺血和缺血再灌注后，ＡＣ活性及ｃＡＭＰ含量均明显增高（Ｐ＜０．０５，Ｐ＜０．０１），缺血心肌ＡＣ活性明显高于再灌注心肌（Ｐ＜０．０５）；静脉注射纳络酮能明显降低缺血再灌注心肌ＡＣ活性和ｃＡＭＰ含量（Ｐ＜０．０５，Ｐ＜０．０１），但对生理盐水治疗的动物正常（模拟手术）、缺血和再灌注心肌匀浆ＡＣ活性无明显作用（Ｐ＞０．０５）。提示，纳络酮本身对ＡＣ活性无抑制作用，其降低缺血再灌注心肌ｃＡＭＰ含量可能涉及酶前因素。
The effect of naloxone on myocardial adenylate cyclase (AC) activity and cAMP content was observed by canine myocardial ischemia-reperfusion model and the effects of naloxone on myocardial AC activity influences. The results showed that AC activity and cAMP content were significantly increased after myocardial ischemia and ischemia / reperfusion (P <0.05, P <0.01), AC activity of ischemic myocardium was significantly higher than that of reperfusion myocardium .05). The intravenous injection of naloxone could significantly decrease the AC activity and cAMP content of myocardial ischemia-reperfusion (P <0.05, P <0.01), but the normal saline treated animals (simulated surgery) Blood and reperfusion myocardial homogenate AC activity had no significant effect (P> 0.05). It is suggested that naloxone itself has no inhibitory effect on AC activity. It may be related to pre-enzyme factors by reducing cAMP content in ischemia-reperfusion myocardium.