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[目的]探讨非甾体抗炎药赖氨匹林(Aspisol)对小鼠黑色素瘤细胞株B16的增殖抑制作用及其可能的机制。[方法]应用MTT比色法分析细胞生长抑制作用,流式细胞技术测定凋亡率,应用Western blot分别检测COX-2蛋白和凋亡相关蛋白Bcl-2、Bax的表达。[结果]2～8mmol/L赖氨匹林可抑制B16细胞的生长,且其作用具有剂量和时间依赖性(P<0.01)。赖氨匹林(2、4、8mmol/L)作用24h后诱导B16细胞的凋亡率分别为5.05%±1.23%、9.25%±1.46%、14.80%±1.98%,并呈浓度依赖性;与对照组(0.18%±0.13%)比较差异有显著性(P<0.01)。赖氨匹林抑制B16细胞COX-2蛋白的表达(P<0.05);赖氨匹林可促进Bax蛋白表达(P<0.05),但抑制Bcl-2的表达(P<0.05)。[结论]赖氨匹林通过影响COX-2蛋白的表达,调节凋亡相关蛋白表达,抑制B16细胞增殖。
[Objective] To investigate the inhibitory effect of non-steroidal anti-inflammatory drug Aspisol on melanoma cell line B16 and its possible mechanism. [Methods] MTT assay was used to analyze the inhibition of cell growth, flow cytometry was used to determine the apoptosis rate, and the expression of COX-2 protein and apoptosis-related proteins Bcl-2 and Bax were detected by Western blot. [Results] 2-8 mmol/L lysine hydrochloride inhibited the growth of B16 cells, and its effect was dose- and time-dependent (P<0.01). The apoptotic rate of B16 cells induced by lisaprin (2, 4, and 8 mmol/L) for 24 h was 5.05%±1.23%, 9.25%±1.46%, and 14.80%±1.98%, respectively, and it was concentration-dependent; The difference between the control group (0.18%±0.13%) was significant (P<0.01). Lysinepirin inhibited the expression of COX-2 protein in B16 cells (P<0.05). Lysipirapril promoted the expression of Bax protein (P<0.05), but inhibited the expression of Bcl-2 (P<0.05). [Conclusion] Lysinepirin can regulate the expression of apoptosis-related proteins and inhibit the proliferation of B16 cells by affecting the expression of COX-2 protein.