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膀胱癌细胞系ＢＴ５６３７经醋酸棉酚或高能震波单独作用和两者联合处理后，癌细胞生长增殖受抑制，分裂指数及集落形成率下降。３Ｈ－ＴｄＲ掺入减少。Ｆｅｕｌｇｅｎ染色后经显微分光光度计检测显示ＤＮＡ含量降低。流式细胞计分析发现醋酸棉酚组Ｇ０／Ｇ１期比例显著增高，Ｓ、Ｇ２／Ｍ期比例减少；震波组Ｓ期比例下降。本研究提示，醋酸棉酚可阻断癌细胞由Ｇ０／Ｇ１期进入Ｓ期，而高能震波则抑制Ｓ期。电镜观察显示，醋酸棉酚及高能震波对超微结构的影响是多方面的，主要是线粒体的改变。以上各项指标的改变在联合组均较单独处理组更显著。生长曲线分析还揭示，醋酸棉酚对癌细胞杀伤和抑制作用与药物剂量和作用时间有关，而且是可逆的，高能震波冲击后细胞生长暂时受抑制。Ｎｏｒｔｈｅｒｎ印迹杂交分析显示醋酸棉酚可抑制Ｃ－ｍｙｃ基因表达。
After bladder cancer cell line BT5637 was treated with Gossypol Acetate or high-energy shock alone, the growth and proliferation of cancer cells were inhibited, and the mitotic index and colony formation rate decreased. 3H-TdR incorporation decreased. After Feulgen staining, microscopic spectrophotometer showed reduced DNA content. Flow cytometry analysis showed that the proportion of G0/G1 phase in the gossypol acetic acid group was significantly higher, the proportion in the S and G2/M phases was decreased, and the proportion of the S phase in the shock wave group was decreased. This study suggests that gossypol acetate blocks the progression from G0/G1 phase to S phase, while high-energy shock waves inhibit S phase. Electron microscopy showed that the effects of gossypol acetate and high-energy shock on the ultrastructure were many, mainly mitochondrial changes. Changes in the above indicators were more significant in the joint group than in the individual treatment group. The growth curve analysis also revealed that the killing and inhibiting effect of gossypol acetic acid on the cancer cells was related to the dose and action time of the drug, and was reversible, and the cell growth was temporarily inhibited after high-energy shock waves. Northern blot hybridization analysis showed that gossypol acetate can inhibit C-myc gene expression.