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用多重PCR方法分析了HPRT基因的自发和甲基磺酸甲酯(MMS)诱发的缺陷突变型的结构改变,PCR扩增带型表明:21个MMS诱发突变有1个是hprt外显子全缺失,1个外显子5部分缺失,19个突变显示V_(79)野生型的带型,30个自发突变的分析表明没有1个带型与野生型相异。我们进一步分析另1株自发型与野生型相异。进一步分析另1株自发缺失率高达40％的突变型,其突变频率与剂量相关,但缺失突变发生率却减少到6％(2×
Multiplex PCR was used to analyze the spontaneous changes of HPRT gene and the structural changes of the mutants induced by Methyl Methane Sulfonate (MMS). The PCR amplification pattern showed that one of the 21 MMS-induced mutations was the full hprt exon. In the deletion, one exon was deleted in 5 parts, and 19 mutations showed a V. (79) wild type banding pattern. Analysis of 30 spontaneous mutations showed that none of the bands differed from the wild type. We further analyzed the difference between the other strain and the wild type. Further analysis of another mutant with a spontaneous deletion rate as high as 40% was associated with dose-dependent mutations, but the incidence of deletion mutations was reduced to 6% (2×