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应用UV-Vis光谱法 ,研究了在pH =1 . 61的酸性缓冲溶液中 ,牛血清蛋白 (BSA)与 6,6′-(3H-2 1-苯并硫醇-3-叉 )二百里酚 (TB)的相互作用。测得生成复合物的最大吸收峰为 440nm ,与试剂相比蓝移 1 0 5nm。微环境的影响使BSA-TB复合物的最大吸收波长之间红移约 3 0nm。应用平衡透析法、摩尔比法和双波长法进行测定 ,结果表明 ,表观摩尔吸光系数εB=1 . 40× 1 0 4L·mol-1·cm-1,平均结合数n =7,表观结合平衡常数K =2 . 5 9× 1 0 6。研究发现 ,该反应基本符合Scatchard模型 ,认为是BSA与TB之间以静电引力和疏水力综合作用的结果。
Using UV-Vis spectroscopy, the effect of bovine serum albumin (BSA) and 6,6 ’- (3H-2-benzothiophenol-3- Thymol (TB) interactions. The maximum absorption peak of the resulting complex was found to be 440 nm, a blue shift of 105 nm compared to the reagent. The influence of the microenvironment redshifts the maximum absorption wavelength of the BSA-TB complex by about 30 nm. The results showed that the apparent molar absorptivity εB = 1.40 × 104 L · mol-1 · cm-1, the average number of binding n = 7, the apparent Combined with the equilibrium constant K = 2. 5 9 × 10 6. The study found that the reaction is basically consistent with the Scatchard model, that is, between BSA and TB electrostatic attraction and hydrophobic effect of the combined effect.