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目的探讨BCL6B基因启动子区域甲基化与结肠癌的关系。方法选取在广州军区武汉总医院2013年1月至2015年1月经病理检查确诊为结肠癌53例患者的癌组织和癌旁组织,来自该院实验室的结肠癌细胞株和正常人外周血淋巴细胞及正常结肠黏膜组织。比较结肠癌组织、癌旁组织和正常结肠黏膜组织BCL6B甲基化状况的差异;比较结肠癌组织和癌旁组织中BCL6B表达和免疫染色评分的差异。分析各病理参数对结肠癌组织BCL6B甲基化的影响。结果 53例结肠癌组织中41例发生BCL6B启动子区域甲基化,甲基化率为77.36%;结肠癌细胞系RKO、HT29为完全BCL6B甲基化,其余为半甲基化;正常结肠黏膜组织未发现甲基化。53例结肠癌组织中有45例(84.91%)BCL6B低表达或表达缺失;53例癌旁组织中有9例(16.98%)BCL6B低表达或表达缺失,两种组织BCL6B表达的差异具有统计学意义(P<0.05);结肠癌组织的染色评分低于癌旁组织[(4.35±2.15)分vs(6.28±3.46)分,P<0.05]。结肠癌组织BCL6B甲基化率在有淋巴结转移、高TNM分期、低分化程度患者中较无淋巴结转移、低TNM分期、高分化程度患者中明显增高,差异有统计学意义(P均<0.05)。结论结肠癌组织BCL6B启动子区域甲基化高于癌旁组织和正常结肠黏膜组织,BCL6B甲基化与淋巴结转移、TNM分期、分化程度相关。
Objective To investigate the relationship between methylation of BCL6B promoter region and colon cancer. Methods Fifty-three patients with colon cancer confirmed by pathological examination from January 2013 to January 2015 in Wuhan General Hospital of Guangzhou Military Command were included in this study. The colon cancer cell lines and normal human peripheral blood lymphocytes Cells and normal colon mucosa. The differences of methylation status of BCL6B between colon cancer tissues, adjacent non-cancerous tissues and normal colon mucosa tissues were compared. The differences of BCL6B expression and immunostaining score between colon cancer tissues and adjacent tissues were compared. The effects of various pathological parameters on the methylation of BCL6B in colon cancer tissues were analyzed. Results The methylation of BCL6B promoter region was detected in 41 of 53 colon cancer tissues with a methylation rate of 77.36%. The colon cancer cell lines RKO and HT29 were completely methylated by BCL6B and the others were hemomethylated. The normal colonic mucosa Tissues found no methylation. There were 45 cases (84.91%) of low expression or expression of BCL6B in 53 cases of colon cancer tissues, 9 cases (16.98%) of 53 cases of paracancerous tissues had low expression or lack of BCL6B expression, and the differences of BCL6B expression between the two tissues were statistically (P <0.05). The staining score of colon cancer tissue was lower than that of paracancer tissue [(4.35 ± 2.15) vs 6.28 ± 3.46, P <0.05]. The methylation rate of BCL6B in colon cancer tissues was significantly higher in patients with lymph node metastasis, high TNM stage and poorly differentiated than those without lymph node metastasis, low TNM stage and well differentiated degree (P <0.05) . Conclusion The methylation of BCL6B promoter region in colon cancer tissues is higher than that in paracancerous tissues and normal colonic mucosa tissues. Methylation of BCL6B is associated with lymph node metastasis, TNM stage and differentiation degree.