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目的:探讨5-氨基乙酰丙酸(5-ALA)通过光动力疗法(PDT)诱导人结肠癌HT-29细胞凋亡的影响及其机制。方法:将人结肠癌HT-29细胞分成4组：空白对照组、5-ALA组、PDT组、5-ALA-PDT组。对照组不给予光敏剂和照光处理，5-ALA组仅给予光敏剂不给予照光处理，PDT组仅给予照光处理不给予光敏剂，5-ALA-PDT组同时给予光敏剂和照光处理。流式细胞术观察HT-29细胞的凋亡情况，逆转录聚合酶链反应(RT-PCR)观察HT-29细胞B型淋巴细胞瘤-2(Bcl-2)及Bcl-2相关X蛋白(Bax)表达情况，利用紫外分光光度法检测含半胱氨酸的天冬氨酸水解酶-3(Caspase-3)表达量的变化。结果:5-ALA-PDT组凋亡率与空白对照组、5-ALA组、PDT组比较，差异均有统计学意义(n P<0.05)。5-ALA-PDT组Bcl-2表达与空白对照组、5-ALA组、PDT组比较，差异均有统计学意义(n P0.05)。5-ALA-PDT组Bax/Bcl-2表达与空白对照组、5-ALA组、PDT组比较，差异均有统计学意义(n P<0.05)。5-ALA-PDT组Caspase-3表达与空白对照组、5-ALA组、PDT组比较，差异均有统计学意义(n P<0.05)。n 结论:5-ALA-PDT可诱导HT-29细胞凋亡，其机制可能与通过Bax/Bcl-2途径诱导细胞凋亡有关。“,”Objective:To explore the effect and mechanism of 5-Aminolevulinic Acid-Photodynamic Therapy (5-ALA-PDT) on the apoptosis of the human colonic carcinoma HT-29 cells.Methods:HT-29 cells were cultured in n vivio and divided into four groups: blank control group, 5-ALA group, PDT group and 5-ALA-PDT group.The control group was not given photosensitizer and light treatment; 5-ALA group was given photosensitizer ; PDT group was given light treatment; 5-ALA-PDT group was given photosensitizer and light treatment at the same time. Flow cytometry was used to observe the apoptosis of HT-29 cells. Reverse transcription polymerase chain reaction (RT-PCR) was used to observe the expression of B-type lymphoma-2 (Bcl-2) and Bcl-2 associated X protein (Bax) in HT-29 cells. Ultraviolet spectrophotometry was used to detect the expression of Caspase-3.n Results:The apoptotic rate of 5-ALA-PDT group was significantly higher than that of blank control group, 5-ALA group and PDT group (n P<0.05). Compared with the blank control group, 5-ALA-PDT group and PDT group, the expression of Bcl-2 in the 5-ALA-PDT group was statistically significant (n P0.05). The expression of Bax/Bcl-2 in 5-ALA-PDT group was significantly higher than that in blank control group, 5-ALA group and PDT group (n P<0.05). The expression of Caspase-3 in 5-ALA-PDT group was significantly higher than that in blank control group, 5-ALA group and PDT group (n P<0.05).n Conclusions:5-ALA-PDT can induce apoptosis of HT-29 cells, and its mechanism may be related to the induction of apoptosis through Bax/Bcl-2 pathway.