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目的研究P53、P63基因在胰腺肿瘤中的表达及其意义;P63基因表达与P53、增殖细胞核抗原(PCNA)、P16表达的关系及其意义。方法通过免疫组化方法对63例胰腺肿瘤和6例慢性胰腺炎手术标本进行了P53、P63、PCNA、P16蛋白的表达研究,探讨它们之间的联系和它们与胰腺癌临床分期、病理分型、预后及转移等生物学特性的关系。结果P63在胰腺导管腺癌中不表达,在腺鳞癌中高表达,6例腺鳞癌有5例P63染色阳性,阳性率为83.3%。P63在腺鳞癌中的高表达伴有胰腺抑癌基因P16的缺失,同时伴有PCNA的高表达,即伴有细胞增殖度的升高,但P63在腺鳞癌中的高表达与P53基因突变无显著相关性。在胰腺组织中可见广泛的胰岛细胞P63染色阳性,染色部位主要位于细胞胞浆。突变型P53蛋白在胰腺癌中的表达与胰腺癌病理分型、临床分期、转移及预后无明显相关性。结论P63基因表达是胰腺腺鳞癌的重要组织学特征,P63的表达在腺鳞癌发病过程中起了重要的作用。P53基因突变是胰腺癌发生发展中的一个重要事件。
Objective To study the expression and significance of P53 and P63 in pancreatic tumors and their relationship with the expression of P53, proliferating cell nuclear antigen (PCNA) and P16. Methods The expressions of P53, P63, PCNA and P16 in 63 cases of pancreatic tumor and 6 cases of chronic pancreatitis were studied by immunohistochemistry. The relationship between them and the clinical stage and pathological type of pancreatic cancer , Prognosis and metastasis and other biological characteristics of the relationship. Results P63 was not expressed in pancreatic ductal adenocarcinoma and highly expressed in adenosquamous carcinoma. P63 was positive in 5 cases of adenosquamous carcinoma, the positive rate was 83.3%. The high expression of P63 in adenosquamous carcinoma accompanied with the deletion of the pancreatic tumor suppressor gene P16 accompanied by the high expression of PCNA accompanied with the increase of cell proliferation. However, the high expression of P63 in adenosquamous carcinoma and the high expression of P53 gene No significant correlation between mutations. In pancreas, a wide range of pancreatic islet cells showed positive P63 staining and the staining sites were mainly located in the cytoplasm. Mutant P53 protein expression in pancreatic cancer and pancreatic cancer pathological type, clinical stage, metastasis and prognosis no significant correlation. Conclusion P63 gene expression is an important histological feature of pancreatic adenosquamous carcinoma. The expression of P63 plays an important role in the pathogenesis of adenosquamous carcinoma. P53 gene mutation is an important event in the development of pancreatic cancer.