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目的:探讨宫颈上皮内瘤变(CIN)中p16及Ki-67表达及其诊断价值。方法:自2014年3~12月选取连州市人民医院48例,广州金域医学检验中心有32例宫颈上皮内瘤变患者为研究对象,其中CINI 28例,CINII 29例,CINIII23例,选择同期30例确诊为慢性宫颈炎或正常的标本为对照组,通过免疫组化技术对所有宫颈组织中的p16及Ki-67进行测定并比较。结果:CINI、CINII、CINIII p16阳性率分别为14.29%、48.28%、91.30%,均明显高于对照组的0.00%(P<0.05)。CINI、CINII、CINIII Ki-67阳性率分别为82.14%、93.10%、95.65%,均明显高于对照组的6.67%(P<0.05)。另外,p16、Ki-67阳性表达随CIN等级增高而上升。结论:p16、Ki-67表达可能参与宫颈上皮内瘤变发生、发展,两者联合测定可作为CIN分级诊断辅助手段。
Objective: To investigate the expression of p16 and Ki-67 in cervical intraepithelial neoplasia (CIN) and their diagnostic value. Methods: From March to December 2014, 48 cases of People’s Hospital of Lianzhou City and 32 cases of cervical intraepithelial neoplasia were studied in Guangzhou Golden Domain Medical Test Center. Among them, 28 cases were CINI, 29 cases were CINII and 23 cases were CINIII. The same period, 30 cases of chronic cervicitis or normal diagnosed as the control group, by immunohistochemistry in all cervical tissue p16 and Ki-67 were measured and compared. Results: The positive rates of CINI, CINII and CINIII p16 were 14.29%, 48.28% and 91.30%, respectively, which were significantly higher than those of the control group (P <0.05). The positive rates of CINI, CINII and CINIII Ki-67 were 82.14%, 93.10% and 95.65%, respectively, which were significantly higher than that of the control group (6.67%, P <0.05). In addition, p16, Ki-67 positive expression increased with the CIN level increased. Conclusion: The expression of p16 and Ki-67 may be involved in the occurrence and development of cervical intraepithelial neoplasia. The combined determination of p16 and Ki-67 may be used as an auxiliary measure for the classification of CIN.