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报道了一种基于表面等离子体共振(SPR)生物传感器的高灵敏检测抗癌药物甲氧檗因的新方法.分别在纳米金颗粒和金膜表面修饰富含腺嘌呤(A)的DNA链,当存在甲氧檗因时,由于一个甲氧檗因分子可与4个A碱基相结合,从而使得修饰在纳米金颗粒和金膜表面的DNA形成稳定的双链结构,进而将功能化纳米金颗粒捕获在金膜表面.由于纳米金颗粒与金膜之间的电场耦合效应可增强SPR信号,从而可实现对小分子甲氧檗因的高灵敏、特异性检测.本方法的检测下限低至0.07 pmol/L,相对比色法和荧光法而言,降低了约5～6个数量级.以4种药物(盐酸小檗碱、青霉素G、硫酸庆大霉素、5-氟尿嘧啶)作为对照考察了该传感器的选择性,结果表明该传感器具有较好的选择性.
A new highly sensitive method for detecting the anti-cancer drug, barrubine, based on the surface plasmon resonance (SPR) biosensor, was reported in this paper.Adenine (A) -rich DNA strand was modified on gold nanoparticles and gold films respectively, In the presence of barberine, since one molecule of barberine binds to 4 A bases, the DNA modified on the surface of the gold nanoparticles and the gold film forms a stable double-stranded structure, and the functionalized nano Gold particles captured on the gold film surface due to the electric field coupling between the gold nanoparticles and the gold film can enhance the SPR signal, which can achieve high sensitivity and specificity of the small molecule barberine detection. The detection limit of this method is low To 0.07 pmol / L, compared with the colorimetric and fluorescent methods, decreased about 5 to 6 orders of magnitude.With 4 drugs (berberine hydrochloride, penicillin G, gentamicin sulfate, 5-fluorouracil) as a control The sensor selectivity, the results show that the sensor has better selectivity.