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用羧甲基茯苓多糖（ＣＭＰ）预处理人外周血淋巴细胞（ＨＰＢＬ），在以ＰＨＡ和ＣｏｎＡ为主的复合诱生剂诱导培养２４、３６、４８、７２ｈ采样检测的促诱生组ＴＮＦ－α、ＩＬ－２、ＩＬ－６、ＩＦＮγ效价（ｘ±ｓ）分别为４５６．１±２４．２，４７５．６±１８５．７，７４３３±１１８９．９，５５８３．０±５３５．５ＩＵ／ｍｌ，分别比无ＣＭＰ的常规诱生组效价高１．５，３．８，１．８，１．４倍；在以ＮＤＶ－Ｆ病毒为诱生剂诱导培养２２ｈ采样，并经酸化和中性化后检测的ＩＦＮ－α效价可达２１２７４．２±５５２３．０ＩＵ／ｍｌ，比无ＣＭＰ的常规诱生组高１．２倍，均有显著差异（Ｐ＜０．０１），说明ＣＭＰ对上述五种细胞因子均有明显的促诱生效应。此外，还采用ＣＭＰ促诱生技术，对ＴＮＦ－α、ＩＬ－２、ＩＬ－６、ＩＦＮγ和ＩＦＮ－α细胞因子进行了中试，提出了低成本、高效益的中试工艺路线，有待于在制备天然细胞因子的细胞工程中推广应用
Human peripheral blood lymphocytes (HPBL) were pretreated with carboxymethyl tuckahoe polysaccharide (CMP), and induced by 24 h, 36 h, 48 h and 72 h of induction with PHA and Con A as inducer. The titer (x ± s) of α, IL-2, IL-6 and IFNγ were 456.1 ± 24.2, 475.6 ± 185.7, 7433 ± 1189.9, 5583.0 ± 535.5 IU / ml respectively, which were 1.5, 3.8, 1.8 and 1.4 times higher than that of conventional induced group without CMP respectively. After inducing with NDV-F virus as inducer for 22h, The titer of IFN-α detected after neutralization was up to 21274.2 ± 5523.0 IU / ml, which was 1.2 times higher than that of conventional induced group without CMP (P <0.01) CMP on the above five kinds of cytokines have a significant stimulatory effect. In addition, CMP-induced technology was also used to pilot test TNF-α, IL-2, IL-6, IFNγ and IFN-α cytokines, and proposed a low cost and high efficiency pilot process route, In the preparation of natural cytokines in cell engineering applications