Dopamine is an important neurotransmitter, and nonenzyme electrochemical sensor of dopamine detection based on highly active material is urgently demanded. In this work, an electrochemical sensor with high sen-sitivity and selectivity based on self-rolled TiO2 microscroll/graphene composite was developed and validated for dopamine sensing. The device exhibited a superior performance for dopamine detection with a detection limit of 4.25 × 10-9 mol L-1, and in the dopamine concentration range of 0.06–90 μmol L-1, the oxidation currents increased linearly with the concentration. The remarkable performance enhancement was mainly as-cribed to the increased surface area due to the porous surface of the TiO2 nanomembrane and highly conductive graphene therein. In addition, we have demonstrated that the sensor could be effectively used on detecting dopamine concentrations in urine samples. Our work demonstrates that the current microscroll-based device is promising in the field of real-time health monitoring for future human community.