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Objective:The aim of the study was to establish models of Hep-2 laryngeal cancer cell line of different oxygen supplying,trying to investigate the impact of normoxia,hypoxia,reoxygenation after hypoxia on apoptosis and expression of proteins HIF-1α and p53 to Hep-2 human laryngeal cancer cell line induced by 60Co γ-ray.Methods:Human laryngeal cancer Hep-2 cells were divided into 3 groups:group A(normoxia),group B(hypoxia),and group C(reoxygenation after hypoxia).All of the cells were exposed to 5 Gy dosage of γ-ray.Flow cytometry(FCM) was used to measure the protein levels of HIF-1α and p53 and to detect cell apoptosis.The protein levels of HIF-1α and p53 were also determined by immunohistochemistry and Western blotting.The expression of HIF-1α mRNA was determined by RT-PCR.Results:The protein levels of HIF-1α and p53 were evidently increased in group B compared to group A.The protein levels of HIF-1α and p53 in group C were lower compared to group B;the rate of apoptosis in group C was higher than that in group B.Conclusion:Hypoxia decreased the effect of apoptosis induced by 60Co γ-ray in Hep-2 human laryngeal cancer cell line.The apoptosis pathway maybe related to some other genes or proteins but not p53 in the conditions of hypoxia and reoxygenation after hypoxia.
Objective: The aim of the study was to establish models of Hep-2 laryngeal cancer cell line of different oxygen supplying, trying to investigate the impact of normoxia, hypoxia, reoxygenation after hypoxia on apoptosis and expression of proteins HIF-1α and p53 to Hep -2 human laryngeal cancer cell line induced by 60Co γ-ray. Methods: Human laryngeal cancer Hep-2 cells were divided into 3 groups: group A (normoxia), group B (hypoxia), and group C (reoxygenation after hypoxia). All of the cells were exposed to 5 Gy dosage of γ-ray. Flow cytometry (FCM) was used to measure the protein levels of HIF-1α and p53 and to detect cell apoptosis. The protein levels of HIF-1α and p53 were also determined by immunohistochemistry and Western blotting. The expression of HIF-1α mRNA was determined by RT-PCR. Results: The protein levels of HIF-1α and p53 were evidently increased in group B compared to group A. The protein levels of HIF-1α and p53 in group C were lower compared to group B; the rate of apoptosis in gr oup C was higher than that in group B. Conclusions: Hypoxia decreased the effect of apoptosis induced by 60Co γ-ray in Hep-2 human laryngeal cancer cell line. The apoptosis pathway maybe related to some other genes or proteins but not p53 in the conditions of hypoxia and reoxygenation after hypoxia.