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目的建立HPLC法测定肝血管瘤活血胶囊中栀子苷的含量。方法采用Nucleodur C18Pyramid(250 mm×4.6 mm,5μm)色谱柱;流动相:乙腈-水(12∶88);检测波长:238 nm;流速:1.0 ml·min-1;柱温:30℃。结果栀子苷在8.55~68.4μg·ml-1范围内线性关系良好(r=0.9998),平均加样回收率为101.6%(n=9),RSD为1.6%。结论本方法操作简便、结果准确,专属性强,可用于肝血管瘤活血胶囊中栀子苷的含量测定。
Objective To establish an HPLC method for the determination of geniposide in Hepatic Hemangioma Capsule. Methods Nucleodur C18Pyramid (250 mm × 4.6 mm, 5 μm) column was used. The mobile phase was acetonitrile-water (12:88). The detection wavelength was 238 nm. The flow rate was 1.0 ml min-1. The column temperature was 30 ℃. Results Geniposide showed good linearity (r = 0.9998) in the range of 8.55 ~ 68.4μg · ml-1 with an average recovery of 101.6% (n = 9) and a RSD of 1.6%. Conclusion The method is simple, accurate and specific. It can be used for the determination of geniposide in Hemangioma Capsule.