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种子固有细菌是植物内生细菌的重要来源,对植物的健康以及接种细菌的定殖能产生重要影响。该文以杂交水稻(Oryza sativa)种子为研究对象,比较研究了不同品种水稻种子中固有细菌群落的多样性。利用799f和1492r这对引物成功地从水稻种子中扩增出固有细菌16SrDNA片段;通过构建16SrDNA文库和扩增核糖体RNA基因酶切分型(ARDRA)的方法,对杂交水稻‘丰优611’(‘丰源A’ב远恢611’)、‘金优611’(‘金23A’ב远恢611’)和‘金23A/09H013’(‘金23A’ב09H013’)3个组合的子代及其各自亲本的种子固有细菌群落结构的多样性进行了研究。构建的7个克隆文库中,每个文库含有200–300个克隆,30–40个操作分类单元(OTU),对ARDRA分型得到的代表序列进行分析,在16SrDNA文库中发现多种细菌类群,包括α变形杆菌(α-Proteobacteria)、β变形杆菌、γ变形杆菌、放线菌(Actinobacteria)、厚壁菌(Firmicutes)和拟菌(Bacteroidetes),优势菌属是泛菌属(Pantoea)和芽孢杆菌属(Bacillus)。不同品种的水稻种子固有细菌群落结构不同,而杂交子代种子中的优势菌与亲本种子中的优势菌在种类和数量上都具有一定的相关性。此外,子代种子中丰度5%以上的细菌也能在各自父本或母本中检测到。
Seed inherent bacteria is an important source of plant endophytic bacteria, plant health and inoculation of bacteria have a significant impact on colonization. In this paper, the seeds of hybrid rice (Oryza sativa) were studied, and the diversity of indigenous bacterial communities in different varieties of rice seeds was comparatively studied. By using 799f and 1492r primers, the 16SrDNA fragment of indigenous bacteria was successfully amplified from rice seeds. The 16S rDNA sequence of 16S rDNA was amplified and amplified by ribosomal RNA digestion (ARDRA) (’Fengyuan A’ ’Yuanhui 611’), ’Jinyou 611’ (’Jin 23A’ ’Yuanhui 611’) and ’Jin 23A / 09H013’ (’Jin 23A’ × ’09H013 ’) The combinatorial progeny and their respective parents were studied for the diversity of their natural bacterial community structure. Among the 7 clones constructed, 200-300 clones and 30-40 OTUs were found in each library. Representative sequences of the ARDRA typing were analyzed. A variety of bacterial groups were found in the 16S rDNA library. Including alpha-Proteobacteria, beta proteobacteria, gamma-Proteobacteria, Actinobacteria, Firmicutes and Bacteroidetes, and dominant bacteria Pantoea and spores Bacillus. The inherent bacterial community structure of rice cultivars with different cultivars was different, while the dominant bacteria in hybrid progeny seeds had a certain correlation with the dominant bacteria in the parental seed in both species and quantity. In addition, more than 5% of the bacteria in progeny seeds can also be detected in their paternal or maternal parent.