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目的:优选瘰疬膏最佳水煎煮提取工艺。方法:设计3因素3水平9组试验,考察加水量、煎煮时间、提取次数对干膏得率影响;同时选取方中君药玄参的主要有效成分哈巴俄苷作为含量考察指标,采用HPLC法测哈巴俄苷的含量。结果:加水量8倍、煎煮时间1 h,提取3次工艺为干膏得率最高,哈巴俄苷的含量最高。结论:根据试验的结果,同时考虑成本,拟定以加水量8倍,煎煮时间1 h,煎煮3次为最佳提取工艺。
Objective: To optimize the best water decoction extraction process. Methods: The design of three factors and three levels of 9 groups of experiments, study the amount of water, boiling time, the number of extraction on dry extract yield; at the same time select Fang Zhongjun Xuan Sen main ingredient Harpagoside as content index, measured by HPLC Harpagoside content. Results: The amount of water 8 times, boiling time 1 h, 3 times the extraction process was the highest dry extract, the highest content of harpagoside. Conclusion: According to the test results, taking into account the cost at the same time, it is proposed to extract water with 8 times, boiling time 1 h, boiling 3 times as the best extraction process.