在肿瘤的实验研究中,多采用体外长期培养的肿瘤细胞系,但后者在其异质性及某些基本生物学特性方面并不能完全代表人体真正的肿瘤。因此直接采用人体实体瘤细胞或经短期培养进行肿瘤实验研究的工作已经受到重视,且已有一些方法用于实体瘤细胞的分离。本文采用联合酶消化法及氯化铵溶胀两步法,选取适当胃癌手术标本进行癌细胞分离,获得较好效果。 方法与步骤 1.选取新鲜,富含癌细胞的胃癌组织,去除坏死 In the experimental study of tumors, tumor cell lines cultured in vitro for a long time are often used, but the latter cannot completely represent the true tumor of the human body in terms of its heterogeneity and certain basic biological characteristics. Therefore, the direct use of human solid tumor cells or the short-term culture of tumor experimental studies has been paid attention to, and some methods have been used for the isolation of solid tumor cells. In this paper, the combined enzyme digestion method and ammonium chloride swelling two-step method, select suitable gastric cancer surgical specimens for cancer cell isolation, get better results. Methods and Procedures 1. Select fresh, cancer-rich gastric cancer tissue to remove necrosis