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目的：研究稀土元素对药用植物细胞组织培养的作用和意义。方法：以药用植物黄连为材料，研究不同浓度的稀土化合物对愈伤组织鲜重增加和生物碱含量的影响。结果：０．１ｍｇ·Ｌ－１Ｙｂ３＋和１ｍｇ·Ｌ－１Ｅｕ３＋促进愈伤组织的生长，高浓度稀土化合物抑制生长。在不含稀土化合物的培养基上的继代培养中，来源于稀土化合物处理的愈伤组织鲜重增加值均比对照低。稀土化合物影响愈伤组织小檗碱、药根碱和巴马汀含量。结论：低浓度的稀土化合物对黄连愈伤组织的生长具有促进作用，低浓度和高浓度的Ｙｂ３＋和Ｅｕ３＋对黄连愈伤组织后续的继代培养均有不利的影响。Ｙｂ３＋抑制愈伤组织中三种生物碱的积累，０．１ｍｇ·Ｌ－１Ｅｕ３＋有利于药根碱和巴马汀的累积
Objective: To study the effect and significance of rare earth elements on the tissue culture of medicinal plants. Methods: The medicinal plant Coptis chinensis was used to study the effects of different concentrations of rare earth compounds on the fresh weight gain and alkaloid content of callus. Results: 0.1mg · L-1Yb3 + and 1mg · L-1Eu3 + promoted the growth of callus, and high concentrations of rare earth compounds inhibited the growth. In subcultures on medium containing no rare earth compounds, the fresh weight gains of callus derived from rare earth compounds were lower than the control. Rare earth compounds affect the callus content of berberine, jatrorrhizine and palmatine. CONCLUSION: Low concentrations of rare earth compounds can promote the growth of Coptis chinensis callus. Both low and high concentrations of Yb3 + and Eu3 + have adverse effects on the subsequent subculture of Coptis chinensis. Yb3 + inhibited the accumulation of three alkaloids in callus, and 0.1 mg · L-1Eu3 + was beneficial to the accumulation of jatrorrhizine and palmatine