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AIM:To study the effect of angiogenesis inhibitor TNP-470on peritoneal dissemination of colon cancer in nude mice.METHODS:The MTT assay was used to evaluate theinhibitory effect of TNP-470 on human colon cancer cell lineLovo.Lovo cells were injected into the peritoneal cavity ofBABL/C nu/nu mice and the models of peritonealdissemination were developed.Thirty nude mice wererandomly divided into control and TNP-470-treated group.In TNP-470-treated group,TNP-470 was injectedsubcutaneously every other day from day 1 until sacrifice ordeath(30 mg·kg~(-1)).The control group received a shaminjection of the same volume saline solution.RESULTS:In vitro,TNP-470 inhibited the growth of Lovocells,with its IC50 at 2.14×10~2 μg·L~(-1).In vivo,TNP-470demonstrated growth inhibition of tumors.Mice body weightand abdominal circumferences were significantly differentbetween TNP-470-treated group(24.5±3.2 g,7.0±1.1 cm)and control group(29.5±2.1 g,10.3±1.5 cm),P=0.005 andP=0.001.The number of disseminated foci was significantlydifferent between the control group(92.1±20.6)and theTNP-470-treated group(40.3±12.3),P<0.001.The maximalsize of foci was significantly smaller in TNP-470-treated group(3.3±0.7 mm)than that of control(7.3±2.3 mm),P=0.004.Mean survival time was significantly longer in TNP-470-treated group(98.00±12.06 d)than that in control group(41.86±9.51 d),P<0.001.CONCLUSION: Angiogenesis inhibitor TNP-470 might be effective in treating peritoneal dissemination of colon cancer and improve the survival rate of nude mice.
AIM:To study the effect of angiogenesis inhibitor TNP-470on peritoneal dissemination of colon cancer in nude mice.METHODS:The MTT assay was used to evaluate theinhibitory effect of TNP-470 on human colon cancer cell lineLovo.Lovo cells were injected into the peritoneal Cavity of BABL/C nu/nu mice and the models of peritonealdissemination were developed.Thirty nude mice wererandomly divided into control and TNP-470-treated group.In TNP-470-treated group,TNP-470 was injectedsubcutaneously every other day from day 1 Until sacrifice ordeath(30 mg·kg -1).The control group received a shaminjection of the same volume saline solution.RESULTS:In vitro,TNP-470 inhibited the growth of Lovocells,with its IC50 at 2.14×10~ 2 μg·L~(-1).In vivo,TNP-470demonstrated growth inhibition of tumors.Mice body weightand abdominal circumferences were significantly differentbetween TNP-470-treated group(24.5±3.2 g,7.0±1.1 cm) and control group( 29.5±2.1 g, 10.3±1.5 cm), P=0.005 and P=0.001. The number of disse Minated foci was significantly different between the control group (92.1±20.6) and theTNP-470-treated group (40.3±12.3), P<0.001. The maximal size of foci was significantly smaller in TNP-470-treated group (3.3±0.7 mm) Than that of control (7.3±2.3 mm), P=0.004. Mean survival time was significantly longer in TNP-470-treated group (98.00±12.06 d) than that in control group (41.86±9.51 d), P<0.001. CONCLUSION: Angiogenesis inhibitor TNP-470 might be effective in treating peritoneal dissemination of colon cancer and improve the survival rate of nude mice.