直接以克隆的逆病毒载体为模板，在反应管中加入适当比例的α－３２Ｐ－ｄＡＴＰ及其他三种ｄＮＴＰ，以单引物进行线性聚合酶链反应（ＰＣＲ）扩增，标记与扩增同时进行，经过一轮ＰＣＲ（５０次循环），可得到高度特异及高效的单链ＤＮＡ探针。作者已将其成功地应用于人类β－珠蛋白基因表达调控的研究。 Directly using the cloned retroviral vector as a template, the appropriate proportion of α-32P-dATP and other three dNTPs were added to the reaction tube, and single-primer linear polymerase chain reaction (PCR) amplification was performed. The labeling and amplification were carried out simultaneously , After a round of PCR (50 cycles), can be highly specific and efficient single-stranded DNA probe. The authors have successfully applied it to the study of the regulation of human β-globin gene expression.