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以东海原甲藻和中肋骨条藻为研究对象,采用室内单种培养和混合培养,设置不同的氮、磷营养条件,研究了不同营养条件对两种微藻的生长状况和种间竞争参数的影响.结果表明:随着氮、磷浓度的增加,两种藻的最大生物量均呈增加趋势,混合培养中两种微藻的比生长率低于单独培养.在混合培养中,生长前期中肋骨条藻是优势种,随着培养时间的延长,东海原甲藻成为优势种,且优势种发生变化的时间与营养条件有关.混合培养中,东海原甲藻拐点出现时间在0.5～4.9d,中肋骨条藻为0～2.6d,东海原甲藻拐点出现时间晚于中肋骨条藻.在各营养条件下,东海原甲藻对中肋骨条藻的竞争抑制参数β均高于中肋骨条藻对东海原甲藻的竞争抑制参数α,当N为128μmol.L-1、P为32μmol.L-1时,东海原甲藻的竞争能力是中肋骨条藻的3.8倍,两者差异最为明显.
Taking Prorocentrum donghaiense and Skeletonema costatum as the research objects, different nitrogen and phosphorus nutrition conditions were set up by indoor single culture and mixed culture. The effects of different nutrient conditions on the growth status and interspecific competition parameters The results showed that the maximum biomass of both algae increased with the increase of nitrogen and phosphorus concentration.The specific growth rate of the two microalgae in the mixed culture was lower than that in the single culture.In the mixed culture, Skeletonema costatum is the dominant species, and Prorocentrum donghaiense is the dominant species with the extension of incubation time, and the time of dominant species changes is related to nutrition conditions. In the mixed culture, the onset time of Prorocentrum donghaiense appeared in 0.5 ~ 4.9 d, Skeletonema costatum was 0 ~ 2.6 days, and the inflexion point of Prorocentrum donghaiense later than that of Skeletonema costatum. The competitive inhibition parameters of Prorocentrum donghaiense to S. costatum under each nutrient condition were higher than The competitive inhibition parameter α of S. costatum against P. donghaiense was 3.8 times higher than that of S. costatum when N was 128 μmol·L-1 and P was 32 μmol·L-1 The difference is most obvious.